![]() ![]() The paper is published in the journal Science China Life Sciences. plain text emails, the former stands out and captures viewers’ attention. This achievement provides a practical tool for researchers in chromosome engineering and genome rewriting, and is expected to provide more comprehensive guidance and support for chromosome design and synthesis. The study found that there were significant differences in the synthesis difficulties of different chromosomes, and the S-index could quantitatively explain the causes of synthesis difficulties for some gene fragments, providing a basis for chromosome sequence design and synthesis and improving the efficiency and success rate of designer chromosome synthesis. The way your email looks in a user's inbox depends on a wide variety of different factors. Make sure your HTML email is responsive for different screen sizes and devices. The research team proposed a Synthesis difficulty Index (S-index) based on the SHAP algorithm to evaluate and interpret the synthesis difficulties of chromosomes. No matter what method you plan to use to create HTML emails, these best practices will help improve the design, user experience, and deliverability of your emails. The model achieved an AUC (area under the receiver operating characteristic curves) of 0.895 in cross-validation and an AUC of 0.885 on an independent test set in collaboration with a DNA synthesis company, demonstrating a high accuracy and predictive ability. The feature with a positive effect value is highlighted in red, and the feature with a negative effect value is highlighted in blue. D, Force plot for 45,138-45,140 kb sequence of M. The black boxes mark the centromeric satellite of Homo sapiens chromosome 22 and telomeres of synV and synX. B, The difficulties of synthesizing DNA sequences for the different locations within the chromosomes. The heatmap shows the S-index for the different sequences and the color has the same meaning in B and C. coli genome, and to the Sc2.0 project's artificial synthesis of the yeast genome, researchers are constantly advancing in the depth and breadth of genome design and synthesis.Ī, The distribution of DNA sequences with different S-index for the natural and synthetic chromosomes and genomes. From the synthesis of the artificial life JCVI-syn1.0 by Craig Venter's team in 2010, to the rewriting and synthesis of the prokaryotic E. Credit: Science China PressĪrtificially synthesizing genomes has broad prospects in fields such as medical research and developing industrial strains. The white sequences indicate the unanalyzed chromosome sequence. The heatmap indicates the synthesis difficulties for the different fragments, which range from difficult (red) to easy (blue). e, Application of the S-index on a specific chromosome. The feature contributions were quantified according to the global importance scores and local SHAP explanations. C, The XGBoost algorithm utilized to build the classification model and calculate the S-index. Key features were identified from these sequence features by machine learning methods. B, Graphical representations of DNA sequences: repeat, GC content, information entropy and other types of features. The sequences were classified into easy-to-synthesize (blue) or difficult-to-synthesize (red). A, Collection of the DNA sequences obtained from high-throughput synthesis. ![]()
0 Comments
Leave a Reply. |